10/13/2023 0 Comments 10x chromium read length![]() Single-cell Immune Profiling library preparation (v2, sc Immune profiling) This in turn enables sequencing of ADT and HTO at different read depths, otherwise not possible when using TotalSeqB and Feature Barcode Technology. Cell surface protein barcodes (ADT) and hashing tags (HTO) are subsequently generated as separate sequencing libraries. In TotalSeqA-based CITE-seq, antibody tags are captured identically to mRNA via their polyA-tail. Feature Barcode tags are captured via the capture sequence 1 on the 10X 3'mRNA beads and cell surface protein and hashing tags will be combined in one library. Feature Barcode Technology (10X Genomics)ĬITE-seq and Feature Barcode provide access to similar information on cell surface protein and enable hashing.Therefore, the 10x protocol for 3´mRNA can be combined with: Cell hashing may be useful to compensate for technical variability between simultaneously processed samples partially as well as to combine several low-input samples in one reaction. ![]() Full-length mRNA information is not preserved.įor bimodal single-cell analysis, it is possible to combine transcriptome analysis with the expression analysis of cell surface markers (pre-defined by user) or the detection of CRISPR guide RNAs. Suitable for high throughput single cell transcriptome analysis (several 1000s of cells or nuclei per library) with detection of up to and over 2000 genes per cell. We offer library preparations for various single-cell applications Single-cell Gene Expression (3´GEX) library preparation (v3.1, sc mRNAseq) The Chromium system enables the user to analyze up to several thousand of cells or nuclei per library.
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